Sodium hydroxide is used to adjust the ph to 8.

Also >> << novel is a combination of a mixture of bile acids and salts indicator Ricinoleic acid, which can be used on Wednesday in the mechanism shown in addition to the automatic analyzer of microbes, in which the presence of Klebsiella and Enterobacter is snow environment. Detection broth of this invention contains from 2. From 3 to 2. 8% of the nutrients, about 0. 196 to about 0. 204% 3 - (alfa-acetonylbenzyl..)-4-hidroksikumarina and about 0. 049 to 0. 051% 2.4 dinitrofenil hydrazones at. alpha. -Ketohlutarovoyi acid that acts as a biological inhibitor suppresses the growth of E. coli, which usually gives good results in tests for Klebsiella and Enterobacter and are also working to stimulate growth of the organism Klebsiella. 2.4 dinitrofenil hydrazones at. alpha. -Ketohlutarovoyi acid also indicates positive growth of the organism Klebsiella. Nutritious part of Wednesday contains about 4. 5 to 5. 5 g / l Gelysate, approximately 9. 0 to 11. 0 g / l tsellobyoza, from about 9. 0 to 11. 0 g / l inositol, from about 0. 24 to 0. 26 g / L yeast extract, and from about 0. 24 to 0. 26 g / l D-biotin. Gelysate of BBL and gelatin hydrolyzate made by pancreatic digestion, and low in cystine and tryptophan. Suitable replacement Gelysate any source of nitrogen-free sugar. Inorganic nitrogen sources such as ammonium chloride, can also be used. Peptones contain small amounts of sugar can be used. Gelysate goal is to provide nitrogen in the body. Purpose tsellobyoza and inositol is to sugar in the body, which causes sugar fermentation sharp change of pH, which causes the indicator. An indicator of the present invention are sodium and dezoksyholatom Ricinoleic acid. The indicator works by creating a milky-white precipitate in an acidic environment. These changes are observed and recorded the mechanism described in Appendix automatic analyzer microorganisms. Indicator mixture also acts to suppress gram-negative microorganisms. From about 9. 0 to 11. 0 g / l sodium Ricinoleic acid. Sodium hydroxide is used to adjust the pH to 8. Is 0. The essence of the invention lies in the effects of chemical inhibitors, 3 - (alfa-acetonylbenzyl..)-4-hidroksikumarina and 2.4 dynytro phenyl hydrazones in. alpha. -Ketohlutarovoyi acid. These inhibitors act to inhibit the growth of microorganisms without Klebsiella. Growth of E. coli species, gram-positive bacteria and microorganisms that lead to high yield positives using traditional methods for detection of chemical inhibitors inhibited the process which uses an automatic analyzer microorganisms. The concentration of 3 - (. Alpha. Acetonylbenzyl-)-4-hidroksikumarina can be from about 0. 196 to about 0. 204%, and is most effective at 0. 2%. Concentration of 2.4 dynytro-phenyl hydrazones at. alpha. Ketohlutarovoyi-acid can be from about 0. 049 to 0. 051%, and is most effective at 0. 050%. If the concentration of any inhibitor is too low, a higher yield of unwanted false positives occurs. If the concentration is very high, profitability is lower. For preparation of 2. times. medium of 100 ml broth detection, Klebsiella and Enterobacter prepared by dissolving 0. 4 g of 3 - (. Alpha. Acetonylbenzyl-)-4-hidroksikumarina in 98 ml of distilled water mixed with 2 ml of 1 N sodium hydroxide solution. When 3 - (. Alpha. Acetonylbenzyl-)-4-hidroksikumarina dissolved, the following will be strattera dosing added to the solution:

Gelysate - 1. 0 g

tsellobyoza - 2. 0 g

Inositol - 2. 0 g

Yeast extract - 0. 05 grams

Sodium dezoksyholatom - 2. 0 g

D-biotin - 0. 05 grams

Sodium salt ritsinoleinovoy acid - 2. 0 g

2.4-dinitrofenil hydrazones at. alpha. Ketohlutarovoyi-acid - 5 mg

solution was stirred for 1:00. 2. 7 mg Green Diamond is added to a solution. PH brought to 8. 0 by using a drop of sodium hydroxide. Solution filter sterilized. Wednesday at double (2x) the usual concentration for use in wells and card described in the annex named automatic analyzer microorganisms. .